SLU-PP-332 5mg

Important Note

This page is informational and does not authorize use. Peptify clients should complete assessment, disclose medications and health history, and follow the clinician-approved plan only.

Do not start, stop, combine, or change a protocol based only on website content.
Emergency symptoms require urgent medical care, not a website or routine follow-up message.

Quickstart Highlights

SLU-PP-332 is a synthetic small molecule (not a peptide) that acts as a pan-agonist of the estrogen-related receptors ERRα/β/γ — an “exercise mimetic” developed in the Burris/Elgendy lab at Saint Louis University[1][5]. All evidence is preclinical — in mice only. The schedule below reproduces published murine-equivalent regimens (mg/kg-derived for a ~25 g mouse) and is not a recommended human protocol. It is an unapproved research chemical, not a medicine, with no human trials and no FDA approval — presented for research and educational use only.

Add 3.0 mL bacteriostatic water to one 5 mg vial → ~1.67 mg/mL (1,667 mcg/mL), the largest practical dilution for accurate dosing.
1,250–2,500 mcg/day (murine-equivalent, for a ~25 g mouse), split into two divided doses — not a human dose.
At ~1.67 mg/mL, 1 unit ≈ 16.67 mcg; 625 mcg ≈ 37.5 units and 1,250 mcg ≈ 75 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.

Dosing & Reconstitution Guide

A single practical dilution with accurate once-daily dosing, step by step

Murine-Equivalent Schedule (3 mL = ~1.67 mg/mL)

Phase / Week(s)	Dose & Frequency	Volume (U-100 units / mL)
Weeks 1–2	625 mcg (2× daily · 1,250 mcg/day)	37.5 units (0.375 mL) per dose
Weeks 3–8	1,250 mcg (2× daily · 2,500 mcg/day)	75 units (0.75 mL) per dose

Reconstitute: Add 3.0 mL bacteriostatic water to one 5 mg vial → final concentration ~1.67 mg/mL (1,667 mcg/mL).
Murine daily dose: 1,250–2,500 mcg/day (murine-equivalent for a ~25 g mouse), given as two divided doses. This is not a human dose.
Easy measuring: At ~1.67 mg/mL, 1 unit ≈ 16.67 mcg on a U-100 syringe (units = mcg ÷ 16.67). The large 3.0 mL dilution keeps every dose at 37.5 units or more for accurate measuring.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Route & frequency: published murine studies used intraperitoneal administration twice daily (25–50 mg/kg)[1][2]. The daily mcg values above are murine-equivalent (expressed for a ~25 g mouse), not approved human doses. No reputable source supports a human protocol for this compound.

Reconstitution Steps

Draw 3.0 mL of bacteriostatic water into a sterile syringe.

Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution is deliberately large so each dose reads at 37.5 units or more, where U-100 syringe markings are most precise. Avoid freezing the reconstituted solution and protect it from light. SLU-PP-332 is a small molecule, not a peptide.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Quantities below assume an 8–16 week murine-equivalent course with twice-daily administration.

Based on the murine-equivalent maintenance dose of 2,500 mcg/day from one 5 mg vial.
8 weeks: ~25 vials
12 weeks: ~39 vials
16 weeks: ~53 vials
Per day: 2 syringes (twice daily)
8 weeks: ~112 syringes
16 weeks: ~224 syringes
Use ~3.0 mL per 5 mg vial for reconstitution.
8 weeks (25 vials): ~75 mL → 8 bottles
16 weeks (53 vials): ~159 mL → 16 bottles
One for the vial stopper + one for the injection site each administration.
Per administration: 2 swabs (4/day)
8 weeks: ~224 swabs → 3 boxes

Protocol Overview

A concise summary of the murine-based regimen reproduced from published preclinical studies.

▪Mechanism: Pan-agonist of estrogen-related receptors (ERRα/β/γ) that activates an aerobic-exercise gene program and boosts mitochondrial function — reported in mice only, not established in humans[1][2].
▪Published route: Intraperitoneal administration in mice, twice daily (25–50 mg/kg); cycle length was 8 weeks in published studies.
▪Murine dose range: 1,250–2,500 mcg/day (for a ~25 g mouse) in two divided doses — murine-equivalent, not a human dose.
▪Reconstitution: 3.0 mL bacteriostatic water per 5 mg vial gives ~1.67 mg/mL for accurate unit measurements.
▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.

Dosing Protocol

The murine-equivalent dosing framework reproduced from published preclinical studies (not a human protocol).

▪Initial phase (Weeks 1–2): 1,250 mcg/day — 625 mcg per injection, twice daily.
▪Maintenance phase (Weeks 3–8): 2,500 mcg/day — 1,250 mcg per injection, twice daily.
▪Route: Intraperitoneal in published murine models; the doses above are murine-equivalent (for a ~25 g mouse), not human doses.
▪Cycle length: 8 weeks in published studies; some research extends to 12–16 weeks.
▪Frequency & timing: Twice per day at consistent intervals, reflecting published murine regimens.

Storage Instructions

Correct storage is what preserves the compound’s stability and activity.

▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; avoid freeze–thaw cycles of the mixed solution[8].
▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.

Important Notes

Practical points that keep daily administration safe and consistent.

▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
▪Regulatory note: SLU-PP-332 is a WADA-prohibited substance (S4.5 metabolic modulators) and is not FDA-approved; there are no human trials and no established human dose[10].

How This Works

SLU-PP-332 is a synthetic small molecule (not a peptide) that acts as a pan-agonist of the estrogen-related receptors ERRα, ERRβ and ERRγ, with strongest activity at ERRα[1][2]. It was developed in the Burris/Elgendy lab at Saint Louis University.

As an “exercise mimetic,” it engages metabolic pathways normally activated by physical training. In mouse studies, acute administration switched on an aerobic-exercise transcriptional program, increased mitochondrial respiration and fatty-acid oxidation in skeletal muscle, and improved endurance and cardiometabolic measures — without the mechanical stress of actual exercise[3][6].
Additional mouse work has reported reversal of mitochondrial dysfunction and inflammation in aging kidney models, alleviation of metabolic syndrome, and improved cardiac fatty-acid metabolism in heart-failure models[4]. Mouse studies used intraperitoneal injection; some users take it orally, but no oral protocol is established.
Critical caveat: all evidence is preclinical, in mice only. There are no human trials, no FDA approval, and no established human dose for SLU-PP-332. The murine benefits should be read as hypotheses; there is no basis to assume they translate to humans.
SLU-PP-332 is not an approved medicine. It is an unapproved research chemical presented here for research and educational purposes only — not for human consumption.

Lifestyle Factors

Contextual factors relevant to preclinical research with this compound.

▪Exercise context: SLU-PP-332 was studied alongside exercise training in some murine models, suggesting possible synergistic metabolic effects.
▪Diet: Diet composition (standard chow vs. high-fat diet) influenced metabolic outcomes in published mouse studies.
▪Environment: Standardize temperature and housing conditions, which can affect metabolic readouts in research protocols.
▪Handling: Compound stability and consistent handling procedures are critical for reproducible results.

Potential Benefits & Side Effects

What murine studies describe. There is no human evidence; do not assume these effects translate to humans.

▪Metabolic (mice): Enhanced mitochondrial function, increased fatty-acid oxidation and improved glucose tolerance in diet-induced obese mice[2].
▪Cardiac & renal (mice): Ameliorated heart failure via cardiac fatty-acid metabolism, and reversed mitochondrial dysfunction in aging kidney models[3][4].
▪Endurance (mice): Increased treadmill running capacity and muscle oxidative capacity in mice[1].
▪Human safety unknown: No human data on tolerability, pharmacokinetics or adverse effects exist; no clinical trials have been conducted[13].
▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
▪Sport restriction: SLU-PP-332 is a WADA-prohibited substance (S4.5 metabolic modulators) for athletes.

Injection Technique

General subcutaneous technique, following established clinical best-practice guidance[14][15]. Note: published murine studies used intraperitoneal administration; the subcutaneous route is not validated for this compound.

▪Wash your hands well with soap and water.
▪Wipe the vial stopper with an alcohol swab and let it air-dry.
▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
▪Draw the intended dose, then check for air bubbles and push any out.
▪Pinch a skinfold at the chosen site between thumb and forefinger.
▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
▪Press the plunger slowly and steadily until it’s fully down.
▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
▪Return the reconstituted vial to the fridge right away.
▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
▪Watch the site for excess redness, swelling, or signs of infection.

Recommended Source

For high-purity research peptides, we point researchers to Prime Lab Peptides for SLU-PP-332 (5 mg).

▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA. Open source
▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
Shop at Prime Lab Peptides →

References

Reference-derived details for SLU-PP-332 5mg.

SLU-PP-332 (5mg Vial) Dosage Protocol Open source
1 ACS Chemical Biology (2023) Synthetic ERRα/β/γ agonist induces an ERRα-dependent acute aerobic-exercise response and enhances exercise capacity (SLU-PP-332). View Source ↗ Open source
2 J. Pharmacology and Experimental Therapeutics (2024) A synthetic ERR agonist (SLU-PP-332) alleviates metabolic syndrome in mice. View Source ↗ Open source
3 Circulation (2024) Novel pan-ERR agonists ameliorate heart failure through enhanced cardiac fatty-acid metabolism and mitochondrial function (mice). View Source ↗ Open source
4 American Journal of Pathology (2023) Estrogen-related receptor agonism reverses mitochondrial dysfunction and inflammation in the aging kidney (mice). View Source ↗ Open source
5 Cell Metabolism / Burris Lab review Overview of SLU-PP-332 as a small-molecule pan-ERR agonist and “exercise mimetic” (preclinical). View Source ↗ Open source
6 Nature Metabolism / endurance studies SLU-PP-332 increases mitochondrial biogenesis, fatty-acid oxidation and endurance capacity in mice. View Source ↗ Open source
7 Peptide Storage Guide Best practices for storing lyophilized peptides (temperature, humidity and light protection). View Source ↗ Open source
8 Bacteriostatic Water Guidance Bacteriostatic water for injection: multi-dose vial stability and handling. View Source ↗ Open source
9 NCBI Bookshelf Best practices for subcutaneous injection: aseptic technique and site rotation. View Source ↗ Open source
10 WADA Prohibited List Classification of SLU-PP-332 as a prohibited substance (S4.5 metabolic modulators) in competitive sport. View Source ↗ Open source
11 Burris/Elgendy Lab, Saint Louis University Discovery and characterization of SLU-PP-332 as a pan-ERR agonist (synthetic small molecule). View Source ↗ Open source
12 Acta Pharmacologica Sinica (2015) The multiple universes of estrogen-related receptor α and γ in metabolic control and related diseases. View Source ↗ Open source
13 ClinicalTrials.gov Clinical-trial registry: no registered or completed human trials of SLU-PP-332 as of 2025. View Source ↗ Open source
14 Centers for Disease Control and Prevention (CDC) Subcutaneous injection technique: angle, site and no-aspiration guidance. View Source ↗ Open source
15 Subcutaneous Injection Technique (Patient Education) How to administer a subcutaneous injection: clinical technique guidelines. View Source ↗ Open source
16 Prime Lab Peptides SLU-PP-332 (5 mg) supplier — purity specifications and certificates of analysis. View Source ↗ Open source

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